Retinoblastoma is a cancer of the eye that occurs in infants and toddlers and can lead to loss of vision, loss of one or both eyes, and even death. Unlike most tumors, these cannot be biopsied because of the risk of spreading cancer to the rest of the body. In 2017, Jesse Berry, MD, surgeon and ocular oncologist at Children’s Hospital Los Angeles, discovered that fluid removed from the eye during treatment of retinoblastoma contained tumor DNA and functioned as a liquid biopsy—providing information about the tumor and opening the door to earlier diagnosis and treatment. In her lab’s latest research, published in Nature Communications, the team determined that in addition to diagnosis, aqueous fluid also could be used for prognosis—to ultimately guide treatment decisions that will produce better outcomes for children with more aggressive disease.
Because retinoblastoma occurs at the back of the eye, it is not readily observable. The condition is typically discovered when parents notice a glow in their young child’s eye in low light or after taking a flash photograph. Historically, the child would be presumed to have retinoblastoma, which is a genetic condition, and treated without a definitive diagnosis. The treatment paradigm shifted five years ago when Dr. Berry’s research reported tumor genetic material could be found in the fluid taken from the front of the eye —called the aqueous humor. A confirmed genetic diagnosis became possible before any surgical intervention, allowing for earlier and more defined diagnosis and treatment.
According to Dr. Berry, Director of Ocular Oncology and the Retinoblastoma Program at CHLA, her lab’s latest finding—the ability to use aqueous humor for prognosis—is equally significant.
“As a clinician who treats these patients, I was so frustrated because I would see two kids who looked the same clinically and who received the same treatment,” said Dr. Berry. “One child would respond beautifully. But the other child had a more aggressive tumor that didn’t respond to treatment or reoccurred. I felt like there had to be something happening at the cellular level responsible for what we were seeing. We now know that molecular analysis of the aqueous humor can explain those differences.”
